An Invtro Study on Anti-Obesity, Antiradical Activities of Siddha Medicine Nathai Choori Chooranam

Traditional systems of medicine have been in trend for treating numerous ailments in many develop and development countries such as India, China, Japan and Korea, Tavian since age-old time. Siddha system of medicine is one of the ancient traditional system of India and practiced mostly in its southern part of India for treating different diseases including even chronic conditions. Nathai choori chooranam composed of five plant equal composition (Borreria hispida seed, Zingiber officinale Rhizome, Cassia auriculata flower, Anddry fruit and Eleusine coracana seed). Plants offer us bioactive molecules those may serve as safer therapeutics to combat existing new world ageing related diseases, and obesity is the major concern among them. Pancreatic lipase inhibitors from plant sources may prove as promising side effects lacking anti-obesity therapeutics, present study was conceived with the objective of anti-lipase, antiradical and DNA cleavage protector activities of Nathai choori chooranam decoction. Phytochemical constituents were screened quantifications of total phenolics, tannins, flavonoids, were done by taking tannic acid, quercetin, as reference molecules. Antiradical activities were evaluated by using different free radicals (ABTS, lipid peroxidation, metal chelating, superoxide and nitric oxide scavenging activities). Also effect of Nathai choori chooranam decoction on DNA cleavage induced by H2O2 UV-photholysis. The antiradical activity of Nathai choori chooranam decoction was proved to be better than the standard ascorbic acid. Antilipolytic function of Nathai choori chooranam decoction was assessed using porcine pancreatic lipase (PPL; triacylglycerol lipase, EC 3.1.1.3) in an in vitro assay system with p-nitrophenyl palmitate (p-NPP) as a substrate. From the data of the results obtained, maximum percentage of lipase inhibition was shown by Nathai choori chooranam decoction (78.92 %) than standard drug. In addition, they showed a protective effect on DNA cleavage.IC50 value were calculated using the logarithmic regression of the dose-response curve after subtraction of both blank and inherent sample fluorescence values. In all cases, the coefficients of determination of the regression (R2) were greater than 0.95. IC50 are the means ± standard deviations of three determinations.