Annexation of biologically active compounds extracted from plants in the lipid layers of micro bubbles for the localized treatment of diseases

The article deals with the generation of monodisperse micro bubbles using a microfluidic device based on 3D printing. The micro bubbles play a role of carriers of biologically active compounds to act locally in the chosen region having the ultrasound as the drug-releasing agent from a known frequency. The micro bubbles are generated by the passage of gas (nitrogen) through an emulsion consisting of coconut oil, a surfactant, and water, forming individual outer shell layers consisting of sunflower oil. In the development of the work, micro bubbles with an average diameter of 23.50m with a dispersion of 1.1% were produced, which characterizes a population with a high degree of homogeneity. The Lupeol used was isolated from Maytenus acanthophylla (Celastraceae) plant leaves by phytochemical and spectrometric techniques, including methods in liquid chromatography and 1H and 13C magnetic resonance. The natural product Lupeol is recognized for presenting actions against inflammation, antitumor (prostate cancer), arthritis, diabetes, heart disease, kidney, and liver toxicity. The micro bubbles generated by the technique described above will be applied in in vitro assays to evaluate the behavior of tumor cells in the presence of a population of micro bubbles after collapse caused by the presence of a known ultrasonic frequency and intensity, allowing the interaction of diseased cells with the biologically active component.