Synergistic anti-inflammatory activity of ethanolic leaves extract of calotropis gigantean ns achyranthes aspera

Achyranthes aspera & Calotropis gigantean plant are used in folklore medicines for a number of ailments. The freshly collected leaves of above plants were screened. Shade dried & firstly defatted with petroleum ether & then ethanol respectively and the leaf extracts were subjected to various physicochemical studies. The percentage yield of both plant of extract was (5.1%) for Calotropis gigantean & (5.25%) for Achyranthes aspera obtained with ethanol. The obtained extracts separately & in combination were used to carry out anti-inflammatory action. Achyranthes aspera leaf extract also exhibited anti-inflammatory activity. The obtained result showed that the EE at dose of 300 mg/kg showed 28.74% inhibition in carrageenan induced paw oedema, 14.64% inhibition of granuloma dry weight & 11.11% inhibition against formalin induced paw edema. Calotropis gigantean leaf extract also exhibited anti-inflammatory activity. The obtained result showed that the EE at dose of 300 mg/kg showed 34.97% inhibition in carrageenan induced paw edema, 18.82% inhibition of granuloma dry weight & 15.77% inhibition against formalin induced paw oedema. Calotropis gigantean & Achyranthes aspera leaf extract in combination also exhibited anti-inflammatory activity. The obtained result showed that the EE at dose (300+100) mg/kg showed 43.62% inhibition in carrageenan induced paw oedema, 25.81% inhibition of granuloma dry weight & 20.77% inhibition in formalin induced paw oedema.