Anticancer activity of marine cephalopod (s.pharaonis) from gulf of mannar

Anticancer activity of crude and partially purified fractionated ink of S.pharaonis (cuttlefish) was evaluated on HepG2 cancer cell line. Different concentration (125µg, 250µg and500µg) of crude and fractionated ink were prepared and the cell viability was measured using MTT assay. Crude ink exhibit significant cytotoxic activity at all concentration. Cytotoxicity increases as the concentration of the sample increases from 125µg to 500µg. Maximum toxicity of 64% was observed at 500µg/ml concentration and IC 50 value obtained at a concentration of 125µg. Anticancer analysis of protein fractionated ink showed potent cytotoxic activity against Hep G2 liver cell line and the toxicity ranged between 56%- 74%. Analysis of fractionated ink resulted in cell death at a concentration of <125. Results indicate that the extracts had a dose dependent inhibitory effect on the growth of the HepG2 liver cell line and the toxicity was higher in the protein fractionated ink of cuttlefish when compared with crude ink. Therefore proteins of cuttlefish ink can be targeted for therapies.