Ventilator-associated pneumonia (VAP) refers to the development of parenchymal lung infection after a patient has undergone intubation and received mechanical ventilation (MV) for ≥ 48 hours. The Quantitative Research approach was used in this study. Pre-Experimental one group pre-test and post-test research design was selected for this study. The study population consists of all the staff nurses. Out of 300 samples in pre-test 24 (8%) of them had poor knowledge, 174 (58%) of them had average knowledge, 83 (27.7%) of them had good knowledge, 19 (6.3%) of them had Excellent knowledge. There was a significant association found between the level of knowledge and the selected demographic variable such as “monthly income” and “area of experience”.