
Objective:- To measure the level of the vitamin D3. Patients and Methods:- blood is typically collected from 100 patients via venipuncture and more recently “blood spots” from a simple finger prick from the individual being assessed. The serum (from venipuncture) or blood (from blood spot) is isolated and used in the assay being employed. There are then a number of methods to assess 25(OH)D to determine vitamin D status. Results:- One hundred patients were studied, 50 female (50%) and 50 male (50%), and female to male ratio was (1: 1). The age ranged from 10 years to 80 years, with a mean age of 40 years + 5 years. The majority being in the fifth decade of life constituting 27 patients (27%) . Also our study showed that the lower level of VITAMIN D3 is 4 ng /dl and the higher level is 45 ng/ dl and the mean level is 19.4 ng/ dl.