Qualitative phytochemical analysis of Trochomeria dalzielii seed oil was carried out with the aim to provide analytical data of the seed oil for its medicinal and/or nutritional importance. The T. dalzielii seed oil was extracted using Soxhlet extractor. Fatty acids composition of T. dalzielii seed oil was determined by GC–MS technique while the phytochemical analysis was done by standard methods. The fatty acids compositions revealed six bioactive components including; Oleic, Linoleic, Palmitic, Stearic, Eicosanoic, and Behemic acids. Oleic acid (57.95%) is the most abundant and Behemic acid (0.47%) is the less. The result of the phytochemical analysis of T. dalzielii seed oil revealed the presence of flavonoids, saponins, tannins, alkaloids, steroids, resins and glycosides. Several studies have reported the importance of these phytochemicals, and based on these findings, it could be suggested that T. dalzielii seed oil might serve as potential nutraceuticals.