Metagenomic study of rhizospheric soil for expounding pgpr diversity

Metagenomic has proven to be a powerful tool for investigating microbial diversity and functional potential within complex environments. Its application has steadily expanded, particularly in exploring the rhizospheric soil microbiome. The rhizosphere—the narrow region of soil influenced by root exudates— notably contribute in plant growth-promoting rhizobacteria (PGPR), which play essential roles in enhancing plant growth. These beneficial bacteria contribute through various mechanisms such as nitrogen fixation, phosphate solubilization, and phytohormone production. Material and methods: Two soil sample from different rhizosphere were collected from Jeevkar Nagar (garden area) and Ishwar Farm (sorghum field) from two different location of Surat, India. Further Bacterial DNA was isolated, purified and quantified. After library preparation samples was sequenced using Sequencing with ion torrent s5 plus system and analysed using alpha diversity analysis method. Results: In our sample analysis three major group of bacterial community dominate the diversity of particular soil microflora respectively, Proteobacteria (76.89 %), Actinobacteria (12.8) and Firmicutes (2.36 %) with minor presence of other bacterial community. Microbial community taxonomic analysis shows 20, 21 phyla; 40,39 class; 55,52 order; 53,50 family; and 55,51 genus and species in sample 1 and sample2 respectively. Conclusions: This study highlights the effectiveness of metagenomics in uncovering the hidden diversity and functional capabilities of PGPRs in rhizospheric soils. The insights gained pave the way for the development of advanced microbial strategies to promote sustainable agriculture and enhance soil health.