Background: A number of evaluations (at small & and large scale) regarding vaccination against HBV have been carried out but very few of them reflect population opinions. Objectives of Study: To find out knowledge, attitude and practices regarding vaccination against HBV among Doctors of NMC, Multan. Study Design: It was a cross-sectional epidemiological study. Setting: The study was carried out in different surgical, medicine and gynecology wards N.H. Multan, and data was completed and analyzed in department of community medicine N.H. Multan. Duration: Study was conducted from 08th September to 15 September 2017 Study Population: H.Os, PGRS, M.Os of medicine, surgery, and Gynecology wards of N.H, Multan. Exclusion Criteria: Professors and senior registrar of Nishtar Hospital, Multan. Sampling Technique: Convenience sampling Non-Probablity. Sample Size: 350 Results: • Residence of doctors 72.6% urban / 27.4% rural • Age of doctors 58.9% (20-25), 39.1% (26-30), 1.7% (31-35) & 0.3 (>35) • Designation of doctors 60% (H.Os, 38% PGRs, 2% M.Os, and above that. • Knowledge of doctors about route of transmission of HBV infection: 68.3% complete knowledge / 31.7 % incomplete knowledge. • Knowledge about HBV infection : 26% complete knowledge/ 74% incomplete knowledge • %age of doctors tested for Hepatitis B: tested 93.7%/ Not tested 6.3% • Vaccination of doctors against Hepatitis B: vaccinated 92.9% / Not vaccinated 7.1% • Vaccination status of family of doctors against Hepatitis B: vaccinated 76.3%/ Not vaccinated 23.7% • Knowledge about preventive measures: complete knowledge 58.3%/ incomplete knowledge 41.7% Conclusion: From our study we concluded that doctors are now keener about vaccination against HBV. Most of the doctors (PGRs, M.Os) reported better knowledge, keen attitude and positive practices regarding vaccination against HBV.