Infection with Hymenolepis diminuta is rarely reported in humans(Tena 1998). In Eastern India only one case had been reported till now from Odisha (Tadepalli Karuna, 2014). So we are reporting this case of isolation of Hymenolepis diminuta from a 3 year old child who was referred to our laboratory for examination of stool sample. To the best of our knowledge this is the first case of human infection reported from West Bengal (Tadepalli Karuna, 2014).