Isolation and Characterization of Leaves Extract of Vernonia auriculifera

The present study was carried out on the Isolation and characterization of the leaves extract of V. auriculifera because; the plant is used for traditional medicine especially its leaf juice is locally known by blood clotting and healing wounds. In the present study, the powdered leaf of plant was sequentially extracted with organic solvents: petroleum ether, chloroform, chloroform/methanol (1:1), methanol and water respectively. Repeated use of solvent extraction followed by Column Chromatography and Thin layer Chromatography, leaf extract of V. auriculifera yielded one compound which was characterized as (E)-methyl 11-hydroxy-1-(4-hydroxyphenyl) tetradec-7-en-14-onenamide using UV, FT-IR and NMR spectral data and by comparing with literature reports.