Influence of lysozyme on adhesion of Bifidobacterium bifidum 791, Bifidobacterium bifidum LWA-3, Bifidobacterium animalis subsp. lactis BB-12 in experience in vitro on human erythrocytes

Aim: To determine the adhesive properties of strains of Bifidobacterium bifidum 791, Bifidobacterium bifidum LVA-3, Bifidobacterium animalis subsp. lactis BB-12 and the effect of egg lysozyme on their adhesive activity in vitro experiments on human erythrocytes. Material and Methods: The bacterial adhesion and the effect of lysozyme in the concentrations range from 3.13 to 1500 μg ml is determined on human erythrocytes I (0) Rh (+) blood group. The average adhesion index (AAI) was determined - the average number of bacteria adhered on one erythrocyte, "K" - the percentage of erythrocytes adhering the bacteria. Results: B. bifidum 791 is highly adhesive (AAI = 4.1 ± 0.113), B. bifidum LVA-3 is low-adhesion (AAI = 2.43 ± 0.073) and B. animalis BB-12 is medium-adhesiv (AAI =3.16 ± 0.053). The "K" was 65.4 ± 3.13%, 51.6 ± 2.16%, 47.0 ± 2.28%, respectively Lysozyme was enhanced the adhesion of these strains by 21-80.4% in a wide range of concentrations and with different intensity, individual for each strain. "K" was increased with concentrations of lysozyme from 6.3 to 1000 μg / ml, and AAI was increased for B. bifidum 791 at 100 - 1500 μg / ml, the increase of "K" was for B. bifidum LVA-3 at 100-1000 μg ml and the increase of AAI was at 25-1500 μg ml of lysozyme, and for B. animalis BB-12 was "K" at 50-1000 μg/ml and AAI at 12.5-1500 μg/ml. Conclusions: Inclusion of lysozyme in the composition of probiotics can enhance their pharmacological effect.