16S/18S ribosomal deoxyribonucleic acid sequencing, polymerase chain reaction and deoxyribonucleic acid sequencing has played a pivotal role in the accurate identification of bacterial isolates and the discovery of novel bacteria and fungi in explosive laden soil. Ten different Bacterial isolates and three different Actinomycetes belongs to the genera Acinetobacter, Bacillus, Enterobacter, Enterococcus, Staphylococcus, klebsiella, Aspergillus, Coriolopsis were isolated and identified with their 16S and 18S rDNA sequences and deposited in the The GenBank Maryland USA. All the isolates were named after the discoverer P Ravikumar, will be preserved in MTCC, India. Sanger dideoxy sequencing technology was employed and the number of base pairs, the base count of A, T, G and C was also studied. To fully utilise 16S/18S rDNA sequencing of bacteria and fungi in explosive laden soils and their bioremediation, the presence of xplA and xplB and other biodegrading gene/s were to be investigated. Aspergillus sojae PRK2 18S ribosomal RNA gene, with the base count 216 a 184 c 256 g 228 t partial sequence with Accession KJ938684, Version KJ938684.1 GI: 675621795 (bases 1-884) a novel strain present in the explosive laden soil of cracker industry was deposited in the The GenBank Maryland USA is discussed here.