Evaluation the different methods detection of AFB1 in animals feed in Iraq

The 145 samples were collected from different region from Iraq during March 2014 – February 2015, distributed into 55 samples imported and 90 samples local markets include: corn, wheat, malt, soybeans, compete cow's feed and feed additives. The samples were analysis to quality and quantity detection of aflatoxin B1 (AFB1) by using methods of Thin layer chromatography(TLC), High performance liquid chromatography (HPLC) and Enzyme Linked Immune Sorbent Assay (ELISA) techniques. The results from quality detection of AFB1 by TLC showed the 30% of samples were positive, While the quantity and quality detection of HPLC and -ELISA techniques results appeared 44.8 % and 41 % of samples respectively were positive (contaminated with AFB1), furthermore the results showed the corn, complete cow's feed and feed additives samples more contaminated samples with AFB1. The highest concentration of AFB1 found in imported compete cow's feed at 100% were positive with AFB1 ranged from 70.5-86.43 ng/ g in HPLC and 76.1-95.1 ng/ g in ELISA whereas in local samples was found feed additives with high concentration of AFB1 at 75% in HPLC and 50% in ELISA were ranged from 1.1 to 817.9 ng/g and 3.32 to 111.47 ng/g in HPLC and ELISA technique respectively while the soy sample was lesser contaminate. The contamination rate was high in the local samples compared to imported samples. Also the results observed that no significant differences between the HPLC and ELISA techniques, but have significant differences when compared with TLC technique, that may be refer into the sensitivity and specificity of HPLC and ELISA were higher than the TLC.