An effective protocol for the in vitro conservation of an important medicinal plant, aloe vera

Aloe vera belongs to the family Liliaceae, commonly called as 'Burn plant'. In this study the basal medium used for the culture is Murashige and Skoog with sucrose 3% and 0.08% Agar, Growth hormones, 6-benzyl amino purine (BA), 3-Indole butyric acid (IBA), Kinetin (Kn), Adenine Sulphate etc. were added to the basal medium either singly or in various combinations. For shoot proliferation, BA (0-2 mg/L) and Kn (0-1 mg/L) at different concentrations in combination with IBA (0.2 mg/L), and agar (0, 0.8%) used. Aloe barbadenesis propagates vegetatively in its natural state, but propagation is too slow for commercial plant production. Shoot with young leaves was collected from the elite Micro shoots were inoculated on MS basal medium with different concentrations and combinations of BA and Kn (in combination of IBA 0.2 mg/L) for shoot proliferation. In both BA and Kn the plants were free from both fungal as well as bacterial contamination to some extent. After 15 days of inoculation, rooting was 100% in hormone- free medium. In case of hormone- free medium, roots were more thick and elongated, while the roots on hormone supplemented medium were thin and less elongated