Detachment of polymicrobial biofilms on glass surface treated with surface modifiers containing fluorocarbon chain

The purpose of this study was to evaluate the adherence and detachment of bacterial biofilms formed on glass surface modified with 10F2S-3I or FF01 using a PMbiofilm model. Modified and unmodified cover glasses were immersed in buffered McBain medium containing stimulated saliva, and theywere cultured to develop PM biofilms. There were separated six experimental groups (n = 6), designated WC: cover glass washing withCPW, WI: modified with 10F2S-3I cover glass washing with CPW, WF: modified with FF01 cover glass washing with CPW, NC: cover glass without washing, NI: modified with 10F2S-3I cover glass without washing, NF: modified with FF01 cover glass without washing.The modified and unmodified groups were further divided by either gently washing withCPW or without washing. Serial dilutions were conducted of the bacterial cultures, which were then cultured on blood agar medium to count CFU.PM biofilms were easily detached from 10F2S-3I and FF01 modified surfaces by washing with CPW. There were no significant difference of CFU among NC, NI and NF. But WI and WF were significantly lower than in WC. It was concluded that PM biofilms grown on the glass surface modified were easily detached compared with the unmodifiedglass groups.