Cost effective synthesis of angiotensin II and angiotensin iv on a hydrophilic polymer support

One of the important factors for a successful solid phase synthesis is the swelling and solvation of peptide bound resin in the reaction medium.Here a novel GDMA-4VP resin support for solid phase synthesis is prepared by suspension polymerization of 4-vinyl pyridine with glycerol dimethacrylate. The hydrophilicity of the cross-linker provides flexibility and polarity to the support. Swelling studies and stability studies were performed. Resin was functionalized and the resin was found to be quite stable even after vigorous conditions of functionalisation. IR spectrum and SEM were used for the characterization of the resin. The efficiency of the peptide was demonstrated by synthesizing biologically active Angiotensin II and Angiotensin IV peptides. Modified Fmoc chemistry is used for the stepwise addition of amino acids in the peptide chain. The procedure of deprotection, coupling, washing is followed. The reagents used were maximum evacuated to reduce the repeated washings. The peptides obtained were with high yield, minimum usage of solvents and almost 99% purity which was checked by HPLC.