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Comparitive evaluation of the antimicrobial efficacy of six different plant extracts and their synergistic effect against streptococcus mutans and lactobacillus: An invitro study

Author: 
Sumayya Sulthana, I. S., Swapna, S., Dr. Mydhili Mungara, Dr. Sunayana Manipal, Dr. Prabu, D., Dr. Rajmohan, M. and Dr. Savithri
Subject Area: 
Health Sciences
Abstract: 

Background: Chewing of sticks from plants of neem, miswak, liquorice and babul have been used as a method of cleaning teeth in rural areas and as part of tradition in many parts of India. Pine was believed to be used in ancient times for cleaning teeth. The seeds and oil of Black cumin also finds wide use in oral hygiene and treating oral diseases. These plant parts are considered to have medicinal values. Objectives: To assess the efficacy of miswak, neem, liquorice, babul, black cumin and pine extracts for their antibacterial effect also to assess for their probable synergistic effect. Materials and Methods: Twigs of neem, miswak, liquorice, babul chewing sticks ; black cumin seeds and pine leaves were sun dried, ground into coarse powder. 5%, 10% and 50% sterile aqueous solutions were prepared. Two types of methodology was done, aqueous extraction and cold infusion method. The filtrate was inoculated onto blood agar plates containing Streptococusmutans and Lactobacillus and incubated at 37⁰C for 48 h. Results: The six plant extracts were found to inhibit Streptococcus mutansand Lactobacillus. Miswak and babul surfaced as strong antibacterials. The combination of these plant extracts showed significant zone of inhibition Conclusion: Pine doesn’t have earlier adequate research to validate its medicinal effects. Six plant extracts and their combination were effective against bacteria and can be used for preparation of antiplaque agents.

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