The petroleum ether extract of stem bark of Syzygium cumini (L.) was investigated for antimicrobial activity against Fungal isolates Candida albicans, Microsporum audouinii, Trichophyton rubrum, Trichophyton mentagrophytes and the bacterial isolates of Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and Bacillus pumilus by disc diffusion method (Zone of Inhibition in mm at 100 µg / disc). It has been reported that petroleum ether extract of stem bark of Syzygium cumini (L.) showed appreciable inhibition against Candida albicans and Trichophyton rubrum (zone of inhibition 09 mm). GC-MS analysis of Petroleum ether extract of stem bark of Syzygium cumini L. shows the presence of four phytochemical compounds includes Cyclopentasiloxane, decamethyl-, 3-Dodecene (E) (%), 2-hexadecanol (%), 1-(+)–Ascorbic acid(%), 2,6-dihexadecanoate(%).