Aim and Objectives: The aim of this study was to compare the depth of penetration of 3 different root canal sealers into the dentinal tubules, Apexit plus, AH Plus, Roekoseal, Using Scanning Electron Microscopy. Materials and Methods: Thirty freshly extracted single rooted premolar teeth were taken and coronal portions were cut with a diamond disc and the root canal length standardized at 14mm.Then, the teeth were instrumented by using the protaper technique to a size of the F2 insrument at the working length. After preparation, thirty samples were randomly divided into 3 groups of 10 each, Group I (Roekoseal), Group II (AH plus), Group III (Apexit plus) and obturation done with lateral compaction technique. Then the samples were examined under scanning electron microscope and the results are obtained. Results: the mean value of depth of penetration of three root canal sealers at the apical third of the roots are Group-III(Apexit plus)167.80±0.84,Group II (AH plus)155.60±0.89 and Group I (Roekoseal) 116.00±0.71. Group III and Group II showed higher penetration depth values compared to Group I.Conclusion: Apexit plus showed deeper sealer penetration into the dentinal tubules than AH plus and Roekoseal sealer.
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