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In Vitro Plant Regeneration From The Callus Cultures Derived From Hypocotyl Segments Of Brassica Oleraceae L Var Botrytis Cv Early Kunwari

Author:

Theriappan, P1 and Aditya K. Gupta

Subject Area:

Life Sciences

Abstract:

The development of in vitro propagation of plants holds tremendous potential for genetic manipulation of crops and other applications. In the present study, attempts have been made to develop a simple, reliable and reproducible protocol for micropropagation from different explants of Brassica oleraceae L var botrytis. Callusing at a frequency of 100% was observed when hypocotyls segments derived from 6-days-old seedlings of cauliflower were cultured on MS medium supplemented with 2,4-D (1mg/L) and Kinetin (1mg/L). Three months old calli were regenerated at a frequency of 25% when subcultured on MS medium supplemented with just kinetin (1mg/L) alone. Regenerated plants grew well when transferred to soil.

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