In Vitro Plant Regeneration From The Callus Cultures Derived From Hypocotyl Segments Of Brassica Oleraceae L Var Botrytis Cv Early Kunwari

The development of in vitro propagation of plants holds tremendous potential for genetic manipulation of crops and other applications. In the present study, attempts have been made to develop a simple, reliable and reproducible protocol for micropropagation from different explants of Brassica oleraceae L var botrytis. Callusing at a frequency of 100% was observed when hypocotyls segments derived from 6-days-old seedlings of cauliflower were cultured on MS medium supplemented with 2,4-D (1mg/L) and Kinetin (1mg/L). Three months old calli were regenerated at a frequency of 25% when subcultured on MS medium supplemented with just kinetin (1mg/L) alone. Regenerated plants grew well when transferred to soil.