The increasing cost of feed ingredients necessitate improving poultry feed utilization to enhance production. To fulfill increased world food demand and to overcome the consequences related to natural and industrial changes, scientists have been trying to select and improve both feedstuff and livestock with the application of biotechnology. The trends of biotechnology application in livestock involve traditional breeding, biochemical and physical techniques in nutrition to increase the abundance (availability) of feed and to improve the utilization of nutrients in those feeds to maximize animal production. Additionally, animal nutrition studies are conducted to determine the safety of human food and modeling of some human diseases. Manipulating animal ability to absorb and utilize more nutrients starts from the plant breeders and continues until where those nutrients are utilized in the body. Microorganisms that have symbiotic life with livestock organism are also transgenically manipulated to improve nutrition and feed utilization. The different biotechnological applications used in poultry industry to improve poultry nutrition and feed includes; engineering of bird’s genes, micro flora of birds and their enzymes, use of antibiotics, Probiotics, prebiotics and others. Hence, biotechnology application is paramount in poultry nutrition and feed improvement for efficient utilization and sustainable development of the poultry industry in the future.