Synthesis of heavy metal nanoparticle by biotechnological approaches for environmental application

The present study is based on the synthesis of silver nanoparticles (Ag NPs) by “cleaner and greener” approach for environmental clean-up. Our way of Green synthesis methods include hydrolysis of protein solid waste, extraction of bio molecule, Synthesis of silver nanoparticle and anti-bacterial application. Proteolysis bacteria isolated from marine environment was identified as Bacillus sp. CJ2 and used for the hydrolysis of protein solid waste, which resulted in bio-molecule rich broth production. From the bio-molecule rich broth, enzymes were separated out by ammonium sulphate precipitation method. Enzyme molecular weight was found to be around 60KDa. Later remaining bio-molecule was utilized for synthesis of silver nanoparticle. Synthesized Nano-particle was found to be stable, which shows the presence of both reducing and stabilizing agent in bio-molecule. The mechanism of the Ag NP bactericidal activity is discussed in terms of Ag NP interaction with the cell membranes of bacteria. The property was found to be positive for the sewage water isolated E coli.