The study was aimed at managing the vast amounts of wastes of the redfish Lutjanus sebea to isolate an industrially useful enzyme-protease. The visceral organ wastes of fish were collected, homogenized with Tris-HCl buffer and precipitated with varying concentrations of ammonium sulphate, acetone and ethanol and was then purified by dialysis and Sephadex G-100 column chromatography. The purification profile (the protein content, protease activity, specific activity, purification fold, and recovery %) were studied in the crude and partially purified samples. The results showed that the activity was maximum when precipitated with 40-60% ammonium sulphate, 100% acetone and 40% ethanol. The fractions with the highest specific activity were selected for further purification by dialysis and Sephadex G-100 column chromatography. The molecular weight of the isolated enzyme was determined by SDS-PAGE and Native PAGE and was to be found 27 kDa. The presence of protease was confirmed by zymography.
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