Contamination of insecticides of different groups were evaluated in the fish species Mystus seenghala in Kharun river (near Atari village Nandanvan Raipur), Chherikheri pond (rural pond) and Navagaon pond (urban pond) of Raipur district collected during the two years months of March to June 2017 and March to June 2018. Sample collection time is 6.00 am. The fish organs- gills, brain, stomach, intestine and gonads from fish were carefully dissected for determination of insecticides. The insecticidal residues were analyzed by using AAS (atomic absorption spectrophotometer). The selected fish organs of the Mystus seenghala insecticides are not detected.