Blood or blood stain is the evidentiary exhibit which is abundantly encountered in the scene of evidence. Contamination of blood is often seen with substances that are used in day to day life, like person killed in hit and run over by train bares stains both of blood and engine lubricating oils or grease. Or other stains like ketchup stain, stain of tea, juice or cooking oil could be found associated with blood in stains. In this present study five different such materials were considered as materials which were mixed with blood of known blood groups. The grouping examination of such mixed stains were carried out to find out if the above mentioned stains have got any capacity to interfere with absorption elution blood grouping method. After examining four hundred samples comprising of such mixed stains along with blank and control sample, it was concluded that the above mentioned materials cannot interfere the absorption elution blood grouping process