The present investigation deals with the cultural studies, pigment analysis, lipid profiling, total sugar content, extracellular ammonium excretion and molecular characterization of cyanobacterium Aphanothece sp. BTA 9019. The study of the bioactive compounds showed a high and comparable amount of sugar, pigments, chlorophyll, carotenoids, phycocyanin, phycoerythin, allophycocyanin including high protein and ammonia excretion in culture conditions. Total carbohydrates were carried out and this study strain produced high content of carbohydrates. The study also revealed that investigated organism produced good amount of secondary metabolites in culture conditions during decline phase of growth; saturated fatty acid and unsaturated fatty acid i.e. palmitic acid (C16:0) and palmitoleic acid (C16:1) were found high. Furthermore, this alga could be considered as a source of supplement of food, therapeutic agent or for use as an ingredient in cosmetics in the future.