The present study aims the evaluation of antimicrobial potential of methanol extract of Kimia dates and to identify potential natural sources for the synthesis of new drugs to address the growing antimicrobial resistance. The crude methanol extract of Kimia dates, Phoenix dactylifera was extracted and antibacterial activity was evaluated determined by paper disc method showed enterprising potential zone of inhibition of 17mm against Salmonella paratyphi B leading to the further investigation using agar cup method on various standard strains demonstrated excellent zone of inhibition (53mm) against S. typhi. MIC of E.coli and S.aureus found in the range of 0.195mg/ml and MBC in at 2.0mglml. Activity guided fraction was performed and found Ethyl acetate fraction exhibited promising activity with a zone of inhibition 38mm for S.aureus and 35mm for E.coli. Phytochemical analysis reveals the presence of glycoside, saponins, alkaloids, flavonoids and tannins. The potent ethyl acetate fraction was further subjected to characterization of bioactive compounds using HPTLC, followed by bioautography, CHNS, FTIR, LCMS and GCMS analysis revealing the presence of 12-Oleanen-3-yl acetate, (3α) (C32H5202) with probability-75% may be the bioactive compound in the Kimia ethyl acetate fraction, belonging to the class triterpenes, showing a wide range of antimicrobial, anti-diabetic, anti-amylase inhibitor, antioxidant, antibacterial, anti-inflammatory, antitumor activities. The Kimia dates ethyl acetate fraction showed the maximum antioxidant activity of 98.57µg/ml, MIC of antiviral activity is 6.25 mg/ml. The Fruit ethyl acetate fraction shows a strong ability to inhibit and reduce 96.48% infectivity of coli phage and completely prevented bacterial lysis at 30 minutes. The phage inactivation kinetics indicates 65.6% at 20 min of exposure. The fruit fraction showed a strong ability to inhibit the infectivity of coli phage and completely prevented bacterial lysis, which it is hoped will promote research into its potential as a novel antiviral agent against pathogenic human viruses.
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