Introduction: The rationale for conducting research is high frequency of morbidity and mortality among newborn infants due to the infection caused by Pseudomonas aeruginosa. Objective: There was the study of antibiogramm of 117 strains of Pseudomonas aeruginosa. Pathogens were isolated from the different loci (the respiratory tract, mucous eye, umbilical wound, ear, blood, urine) among 117 newborns. Methods: The determination of Pseudomonas aeruginosa’s sensitivity to antibiotics by standard discs method. Results: The 10 strains were nosocomial and referred to the three genetic types (3 strains - I and II type, 4 strains - III type). The 107 clinical strains were individual ("wild-type"). All the strains were sensitive to Piperacillin, Piperacillin + Tasobactam, Colistin and resistant to Carbenicillin, Doxycycline, Cefaclor, Chloramphenicol. The sensitivity to the rest of the 15 antibiotics was variable. Conclusions: The nosocomial and individual strains Pseudomonas aeruginosa are polyresistant to antibiotics. The systematic implementation of microbiological monitoring should be (2-4 times per year) in the stationary and also the antibiotics’ rotation has to be applied.