Selection and screening of highest lignolytic white rot fungus from Warangal District and its molecular identification

Hundred isolates of white-rot fungi were tested for their efficiency of wood degradation based on guaiacol-oxidation, lignin peroxidase, laccase and manganese peroxidase activities. The purpose of the test was to select the best isolates that show high efficiency of producing lignolytic enzymes. Among these isolate BR-1 (Pycnoporus cinnabarinus) was identified as the most good and highest activity for lignin degradation. Further its identification was confirmed in molecular level carried by the internal transcribed spacer ‘ITS’ regions of the ribosomal DNA (28S rDNA) sequencing. The D2 region of LSU (Large subunit 28S rDNA) gene sequence was used to carry out BLAST with the database of NCBI gene bank database.