Aim: The current study was designed to study the toxicological evaluation of Vrikshamla herbal formulation for weight management. Material and method: The whole study also includes the standardization of selected herbal formulation with various parameters like Ash value, extractive value, moisture content, pH and phytochemical investigation. Herein we assessed the broad spectrum safety of Vrikshamla in a battery of in vitro and animal toxicological studies such as, acute oral toxicity studies (1000mg/kg and 2000 mg/kg orally of test sample) was conducted to determine the safe dose as per OECD-423 guideline. The primary skin and eye irritation tests on rabbits classified Vrikshamla drug according to Draize mathod, acute dermal toxicity study in mice. Result: No sigh of toxicity was observed in all parameters except mildly irritating to the eye of rabbit. Conclusion: These results, combined with the tolerability of Vrikshamla in the human clinical trials, demonstrate the broad spectrum safety of Vrikshamla.