Protective effect of Polygonum cognatum Meissn ethanolic extract on experimental hemorrhoids in rats

In this study was aimed to investigate effects on the levels of plasma and rekto-anal tissue some biochemical and histopathological parameters of Polygonum cognatum Meissn extract on hemorrhoid rats. A total of 32 Spraque Dawley female rats, weighing 200-250 g were used in the present study. Thirty two rats were randomly assigned into 4 experimental groups (8 rats per group). They were allowed to adapt to laboratory conditions for 7 days. The study was conducted for 18 days. Rats were divided into the following groups: Control Group, Croton oil Group, Polygonum cognatum Meissn. extract (PCE) Group, Croton oil + Polygonum cognatum Meissn extract Group. After 3 days of croton oil application, rats were treated with Polygonum cognatum Meissn extract for 15 days. Blood and recto-anal tissue samples were collected for biochemical and histopathological studies. Plasma and recto-anal tissue glutathione, catalase, glutathione peroxidase and superoxide dismutase activity were significantly decreased in compaired to Croton oil group. Despite this decrease, plasma malondialdehyde; recto-anal malondialdehyde and myeloperoxidase levels increased in this group. In contrast plasma catalase, glutathione peroxidase and superoxide dismutase activity and recto-anal tissue glutathione, catalase, glutathione peroxidase and superoxide dismutase activity were significantly increased, plasma malondialdehyde; recto-anal malondialdehyde and myeloperoxidase levels decreased in following treatment with PCE. Polygonum cognatum Meissn extract can play an protective role recto-anal tissue damage and oxidative stress that can be concerned with modulating of antioxidant molecule activities in rat of hemorrhoid which can be used safely for the treatment of hemorrhoids in the large population of patients who do not want to undergo surgery and do not want to use drugs or cream due to their many side effects. Polygonum cognatum Meissn extract can play an protective role recto-anal tissue damage and oxidative stress which may be related to modulating the activities of antioxidant molecules in rat model of hemorrhoid.