Background: Increased prevalence of anemia has been observed in adolescent, who are susceptible more often to nutritional anemia. Materials and methods: This retrospective study was conducted on 276 newly admitted MBBS students of Prime Medical College, Rangpur, Bangladesh. For this serum haemoglobin level, total count of RBC and RBC indices of each student were recorded from records preserved in Pathology Department of Prime Medical College. Results: Among 276 male and female students, 33.70% (93) were anemic. Again, 16.66% (22) among the male and 48.61% (71) among the female students were anemic. Mild anemia was observed in 16.66% (22) male and in 31.94% (46) female students and 17.36% (25) female had moderate anemia. The differences among the anemic students were statistically highly significant (p<0.001). Conclusion: The observed prevalence of anemia is more among the female students and most of the students are mildly anemic.