CERTIFICATE

IMPACT FACTOR 2021

Subject Area

  • Life Sciences / Biology
  • Architecture / Building Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology & Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical Systems
  • Physics
  • Social Sciences & Humanities

Why Us? >>

  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly

Prevalence and detection of toxigenic a. flavus, a .niger and a. ochraceus by traditional and molecular biology methods in feeds

Author: 
El-Hamaky, A.M., Atef A. Hassan, Heidy Abo El Yazeed and Refai, M. K.
Subject Area: 
Health Sciences
Abstract: 

A total of one hundred feed samples were collected from Cairo and Giza governorates and screened for aflatoxigenic and ochratoxigenic fungal isolates. A total of 106 fungal isolates comprising, Aspergillus flavus, A. ochraceus and A. niger wererecovered from feed samples and tested for aflatoxins and ochratoxin A (OTA) production. The most predominant isolate was A. flavus, which was recovered at the range of (40-55%), followed by A. niger (30-50 %) and A. ochraceus (15-20%). Thirty three of 47 A. flavus isolates produced aflatoxin B1 and B2 at average levels of (170-750 ppb), while, 22 of 44 tested isolates of A. niger produced OTA with average levels of (100-550 ppb), whereas, 12 of 15 A. ochraceus isolatesproduced OTA at average levels of (300-700 ppb). Molecular identification of 16 toxigenic fungal isolates (5 A. flavus, 6 A. ochraceus and 5 A. niger) was carried out by PCR. The results of PCR of the DNA extracted from these isolates using ITS primer confirmed the identification of A. flavus, A. ochraceous and A. niger. The application of real-time PCR (RT-PCR) system directed against the nor-1 gene of the aflatoxins biosynthetic pathway was applied on the DNA extracted from the 5 selected strains of A. flavus. The amplification plot of the DNA samples indicated the presence of nor-1 gene in all aflatoxins-producing A. flavus isolates and in only one isolate of the negative aflatoxins-producing A. flavus. On the other hand, the use of primer set for amplification of omtB gene responsible for AF production amplified 611 bp fragments bands in all aflatoxigenic A. flavus, while, no band was detected in all negative aflatoxinogenic isolates. All the sequenced A. flavus isolates were confirmed to belong to A. flavus species and were 100% similar to the reference isolates of A. flavus. The application of PCR assays for detection of ochratoxigenic fungi using OCRA1/OCRA2 primers, amplified a single fragment of about 400 bp, when genomic DNA from ochratoxigenic A. ochraceus and non- ochratoxigenic A. ochraceus isolates were tested. No product was observed with genomic DNA from A. niger isolates. When, PCR assayswas applied using a pair of primers (Aopks1/Aopks2) for specific detection of ochratoxigenic fungi by targeting the metabolic pathway genes Polyketide Synthase (pks) specific to ochratoxin, a single fragment of about 549 bp was produced with all positive ochratoxigenic A. ochraceus isolates and 2 ochratoxigenic A. niger isolates. No product was observed with genomic DNA from all negative ochratoxigenic isolates of A. ochraceus and A. niger. Moreover, the combination of biochemical and molecular methods is needed to correctly evaluate the potential toxicological risk in feed caused by these fungi. Conclusion: the application of molecular biology technique was found to be rapid, highly specific, easy to perform and cost effective method to assist creation the programs used for reducing the risk of harmful effects of toxigenic fungi and their toxins to human and other farm animal's health.

PDF file: 

CALL FOR PAPERS

 

ONLINE PAYPAL PAYMENT

IJMCE RECOMMENDATION

Advantages of IJCR

  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High Qualitiy reader base
  • High level author suport

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

 

EDITORIAL BOARD

CHUDE NKIRU PATRICIA
Nigeria
Dr. Swamy KRM
India
Dr. Abdul Hannan A.M.S
Saudi Arabia.
Luai Farhan Zghair
Iraq
Hasan Ali Abed Al-Zu’bi
Jordanian
Fredrick OJIJA
Tanzanian
Firuza M. Tursunkhodjaeva
Uzbekistan
Faraz Ahmed Farooqi
Saudi Arabia
Eric Randy Reyes Politud
Philippines
Elsadig Gasoom FadelAlla Elbashir
Sudan
Eapen, Asha Sarah
United State
Dr.Arun Kumar A
India
Dr. Zafar Iqbal
Pakistan
Dr. SHAHERA S.PATEL
India
Dr. Ruchika Khanna
India
Dr. Recep TAS
Turkey
Dr. Rasha Ali Eldeeb
Egypt
Dr. Pralhad Kanhaiyalal Rahangdale
India
DR. PATRICK D. CERNA
Philippines
Dr. Nicolas Padilla- Raygoza
Mexico
Dr. Mustafa Y. G. Younis
Libiya
Dr. Muhammad shoaib Ahmedani
Saudi Arabia
DR. MUHAMMAD ISMAIL MOHMAND
United State
DR. MAHESH SHIVAJI CHAVAN
India
DR. M. ARUNA
India
Dr. Lim Gee Nee
Malaysia
Dr. Jatinder Pal Singh Chawla
India
DR. IRAM BOKHARI
Pakistan
Dr. FARHAT NAZ RAHMAN
Pakistan
Dr. Devendra kumar Gupta
India
Dr. ASHWANI KUMAR DUBEY
India
Dr. Ali Seidi
Iran
Dr. Achmad Choerudin
Indonesia
Dr Ashok Kumar Verma
India
Thi Mong Diep NGUYEN
France
Dr. Muhammad Akram
Pakistan
Dr. Imran Azad
Oman
Dr. Meenakshi Malik
India
Aseel Hadi Hamzah
Iraq
Anam Bhatti
Malaysia
Md. Amir Hossain
Bangladesh
Ahmet İPEKÇİ
Turkey
Mirzadi Gohari
Iran