A pioneering study was conducted to reveal the spider diversity in surroundings of river Narmada in central India. The objectives of the present study were to explore the diversity and abundance of spider fauna at different habitats. In total 26 species of spiders belonging to 10 families were observed. Thus family Araneidae was the most dominant family exploring 35% of species. The Narmada Valley area is home to several species rich endemic flora and harbors different species of fauna. The area prevail humid and moderate rainfall and climate. The high species diversity of spiders in river Narmada surrounding can be attributed to the high diversity of plants and insects.