The behaviour paradigms are relatively complicated, it is necessary to understand how the fundamental behaviour is organized at neural level, before a full understanding of the complex behaviour. Drosophila melanogaster has shown biased preference when facing sensory stimulation towards varied concentration of stimulants namely nicotine and caffeine. The preference behavioural assays were used to study sensory abilities based on feeding behaviour and climbing ability. The regulation of feeding behaviour in pre- adult and post- adult traits of Drosophila melanogaster showed varied responses to the stimulants supplemented in the food regimes with respect to various concentrations of stimulants addicted with organismal stress provided in the form of starvation. The pre-adult (larvae) preferred stimulants (i.e., Caffeine/Nicotine) rather than control and combination of both the stimulants, while the post-adult (flies) preferred the combination of stimulants than the caffeine or nicotine alone.