Objectives: Evaluation of Physical and Chemical quality of drinking water available in Educational institutes of city Hyderabad of Sindh Pakistan near Indus River. Background: Drinking water quality is the great public health concern because it is a major risk factor for various viral, bacterial and protozoan diseases, globally. Over 25 million deaths are blamed due to waterborne-diseases. Pakistan has been facing the same problem due to improper water management, bad sanitary condition and poor drinking water quality. Human activities also contribute further more impurities and ultimately it affects the quality of water. If this contaminated water is used for drinking water, it may cause health problem. The educational progress of students depends on health. The water is one of the vital components of human health, because about 60-65% diseases are spread through water. Study design: Cross sectional study Study setting: Thirty two Educational Institutes randomly selected in the field practice area of Department of Community Medicine, LUMHS, Water Testing & Surveillance Laboratory, LUMHS, Jamshoro, Sindh. Material and Methods: The drinking water samples from Thirty two Educational Institutes located in Hyderabad were collected as per standard procedure. Analyzed from various physico-chemical parameters and Microbiological parameters as per PSQCA(Pakistan Standard Quality Control Authority)/ WHO standards. For analysis samples were transported to Water Testing & Surveillance Laboratory, Department of community medicine, LUMHS, Jamshoro, Sindh. Results: Data revealed that the pH of all samples ranged from 7.7-7.9, conductivity 750 µS to 879µS, Salinity 0.5 %, Total Dissolved Salts 592mg/L to 736mg/L, The Turbidity of sample No: 2, 15,25,26,27,28,29,30,31 & 32 is higher than the permissible limit of WHO i. e; 5 NTU, Arsenic level of all samples shows within the limits of WHO standards, while Total coliform, Total Fecal Coliform and E-Coli were positive in all the samples.