Objective: To evaluate the level of knowledge of periodic health examination among teachers and, therefore, to assess the need to increase the knowledge of and training on PHE. Method: Cross-sectional, analytical study. Conducted throughout September 2019. The study involved 18 secondary schools, selected randomly from the 10 different educational sectors, in Makkah al-Mukarrama. Our total participants were 214 male teachers. Results: More than half of the participants (55.7%) had “weak” knowledge of PHE, 41.4%of the participants had no knowledge of PHE, and only 3.0% had average knowledge. Conclusion: More effort is needed to ensure that periodic health examination is practiced. There is a need to study the factors associated with the uptake of periodic health examination (PHE) among the study population.