The Democratic Republic of Timor-Leste will establish bonds of friendship and cooperation especially with neighboring countries in their respective regions” and viewed from history and geographically. Lack of support of the government to improve agricultural productivity and industries and human resources a capacity to compete in any market employment and business activity in the region. These indicator became the opposite idea for Timor-Leste to join as an ASEAN member, in terms of economic aspects, especially agriculture and industry. This research aimed to analyze and find out the opportunity and challenges in preparing Timor-Leste to join ASEAN in agriculture and industry sector. The method in this study is a qualitative method with a descriptive approach, the type data and data collection techniques namely observation, interviews and documentation, and data analysis techniques using the model Milles and Humberman. Base on the statistical analysis shows the gross domestic product (GDP) of the ASEAN countries from 2008 to 2016, with projections up until 2018. The estimated GDP of all ASEAN states amounted to approximately 2.57 Trillion American Dollars and it can be argued that Timor-Leste economic contribution has declined compared to ASEAN member countries.