The cold-tolerance of ‘Kristal’ (Euro-Amur-American origin), ‘Dostoyniy’ and ‘Krasnostop AZOS’ (interspecific hybrids of Euro-American origin) grapevine varieties in the conditions of the Russian South winter is characterized by the second (true dormancy) and third (induced dormancy) winter-resistance components. Here we used complex approach to estimate the plants adaptability to abiotic stress factors. We used gravimetric method for humidity assessment and water content; spectral analysis for detection of the protein level and pigments; capillary electrophoresis method to analyze the level of carbohydrates, Krebs cycle organic acids, phenolcarbonic, ascorbic acids, and amino acids. The obtained results allow to suppose the various mechanisms of adaptation of the studied varieties to the winter period stressors. The water content of the shoots, the level of protein, aminoacids, proline, sugars and the sum of phenolcarbonic and ascorbic acids are the most informative indicators of grape plants frost-resistance in the climate conditions of Anapa - Taman region.