Isolation, screening of marine actinomycete strain l3 for antibacterial activity and optimization of process parameters for the production of lipase Streptomyces yogyakartensis

Marine sources are increasingly being investigated as a source of microorganisms with potential to produce novel bioactive compounds and enzymes. Marine ecosystems represent a largely untapped source for isolation of new microorganisms. They are of particular interest, since they are traditionally known for their unparalleled capacity to produce biomolecules with diverse biological activities. Actinomycetes were isolated from marine sediments collected off the coast, Visakhapatnam and, Machilipatnam, Andhra Pradesh. The antibacterial and Lipase activities of the strain were determined and fermentation conditions viz., incubation time, incubation temperature, inoculum age, initial pH, were optimized for maximizing the antibacterial metabolite and enzyme production by strain L3. Physiological and Biochemical characteristics of the isolate were studied by the conventional method and identified as Streptomyces Yogyakartensis strain L13 by the Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh. Further the medium composition with respect to carbon and nitrogen sources was also optimized for increasing the antibacterial metabolite and lipase production. With the optimized conditions employed, strain showed antibacterial activity (in terms of inhibition zone diameter) of 38 mm against Enterobacter aerogenes & 39.5mm against Bacillus subtilis and Lipase activity of 41.2 U/ml.