This is the first report on identification of plasma material for development of indigenous HBV standard for nucleic acid techniques. The HBsAg positive plasma bag was collected from National Capital Region. The hepatitis B virus in the plasma was quantitated by using four different lots of two different assays. The mean viral load determined was 5.59 log10 IU/ml. The plasma material was found to have genotype-D of the HBV. The nucleotide sequence was submitted under accession no. LK995378.