High frequency in vitro propagation of banana, cv. karibale

In the present study, an efficient reproducible protocol for high frequency in-vitro propagation of Banana cultivar Karibale was described. An in-vitro protocol for Cv. Karibale was established by using shoot tip culture. The healthy suckers which were collected from the disease free and high yielding mother plants were surfaced sterilized and initiated on standard MS medium supplemented with different combinations and concentrations of Plant growth hormones. Out of tried combinations, maximum multiplication (95%) was obtained in MS medium supplemented with 4 mg/L BAP, 8 mg/L Adenine Sulphate and 0.5 mg/L NAA, while maximum elongation of shoot (4.2cm) was observed in MS medium supplemented with 3.5 mg/L BAP, 6 mg/L Adenine Sulphate and 0.5 mg/L IAA. Then the micro shoots were transferred to in-vitro rooting medium MS medium half strength, supplemented with 1.5 mg/L BAP, 2 mg/L IAA, 1 mg/L IBA and 2 g/L Activated charcoal. Then excellent acclimatization was observed for transferred plantlets with 90% survival rate.