It was became clear that during last 4 billion years, owing to the bioevolution link existed between the two basic electron, proton dependent metabolic reaction systems of obtaining of ATP had been formed the various capacity of ATP based regulation of expansion in the number of genes in the case of the human gene and also in the case of Archea genome and Bacteria genome. We are developing the idea that the evolution based difficulty as the limitation of expansion in the number of genes because of slow developed systems of ADP + Pi + H+ + nH + memb.space, and the unsifficient of membrane redox potentials three - state line system in case of prokaryotes had been solved by appearance of powerful energy delivering systems as “Donators + membrane redox potentials three - state line system + O2 + ADP + Pi + H+ + nH + memb. space = (ATP + heat energy) + H2O + nH + matrix + CO2” (Ambaga and Tumen-Ulzii, 2015), conditioning the high capacity of ATP based increase of Genome Size . It can be say that during evolution development of living cells the shift from one cell to multicells had been accompanied with their metabolic system improvement as first slow developed system as ADP + Pi + H+ + nH + memb.space had converted to second powerful energy delivering system as “Donators + membrane redox potentials three - state line system + O2 + ADP + Pi + H+ + nH + memb.space = (ATP + heat energy ) + H2O + nH + matrix + CO2” (Ambaga and Tumen-Ulzii, 2015), which led to appearance of high capacity of ATP based increase of Genome Size. In such way the appearance of second more powerful energy accumulating systems as “Donators (glucose, aminoacids, fatty acids) + membrane redox potentials three - state line system + acceptor as O2 + ADP + Pi + H+ + nH + memb.space = (ATP + heat energy) + H2O + nH + matrix + CO2” had conditioned the increase the gene size, number of genes, linear gene structures in Human organism in comparision to Archea genome and Bacteria genome.