The Ebola virus (EBOV) genome encodes for several proteins that are necessary and sufficient for replication and transcription of the viral RNAs; NP, VP30, VP35, and L. VP30.VP30 binds to the RNA at the first gene start signal to initiate transcription. In the present study we used different In Silico tools and technique to analyze protein sequence of VP30 protein retrieved from major protein resources, prediction of three dimensional structures and its binding site prediction. However, we predicted the three dimensional structure of VP30 protein by Swiss model server and Ramchandran Plot analysis. Our work suggests that VP30 protein can acts as target for the inhibition of Ebola virus. The further study of VP30 protein used in the molecular docking and structure based drug designing to inhibit in Ebola virus .