The quality of herbal products which varies with the time under the influence of environmental factors, such as temperature, humidity, moisture, other ingredient or excipient in the dosage form, microbial contamination, adulteration, lack of good manufacturing practices, poor quality control procedures, poor agriculture and post harvesting methods. Therefore qualitative and quantitative evaluation of herbal drugs can serve as an important tool for monitoring the safety herbal formulations. The present study was performed quantitative assay, method development and validation of Boswellic acids using HPLC and HPTLC and also its application in various marketed formulations of Boswellia. Method Validation was performed as per the ICH guidelines. From all the validation parameters it is very clear that the HPLC and HPTLC method developed was sensitive, accurate, precise, linear, rugged, robust, and convenient and it could be applicable for quality control studies of marketed formulations of Boswellia.