Effectiveness of benzylaminopurine (BAP) concentrations on In-vitro shoots proliferation

In present study benzylaminopurine efficiency was tested on shoot proliferation efficiencies under in vitro condition. About 45 explants were cultured on each of medium supplemented with different concentrations of 0.0, 0.5, 1.0, 2.0, 3.0 and 4.0 mg/L BAP. Concentration of BAP significantly affected the average of survival rate, shoot numbers, and shoot length per explants. Whereas, MS basal medium supplemented with 2.0mg/L concentration of BAP showed astonishingly survival rate and the maximum number of shoots (16shoots/explant). MS medium supplemented with 4mg/L of BAP followed by 3mg/L of BAP showed optimal efficiency. While, shoots regenerated on MS basal medium (control) without addition of BAP showed significantly lower survival rate (57.86%), and resulted lower number and length of shoots up to the end of experiments.