Introduction: Antenatal care is the systemic medical supervision of antenatal mother during pregnancy period. World Health Organization recommends a minimum of four antenatal visits, comprising interventions such as tetanus toxoid vaccination, screening and treatment for infections and identification of warning signs during pregnancy. In India the maternal mortality ratio has been maintained at a higher level since long. Statement of the problem: “A study to assess the effect of video assisted teaching programme on knowledge and attitude regarding antenatal care among antenatal mothers in primary health center, Kalapet, Puducherry”. Objectives of the study: 1. To assess the level of knowledge and attitude on antenatal care among antenatal mothers. 2. To assess the effect of Video Assisted Teaching Programme on knowledge and attitude regarding antenatal care among antenatal mothers. 3. To correlate the knowledge and attitude on antenatal care among antenatal mothers. 4. To determine the association between level of knowledge and attitude regarding antenatal care among antenatal mothers with selected socio demographic variables. Methodology: An evaluative approach with pre-experimental one group pre-test and post test design was used in the study. The study was conducted in primary health center, Kalapet, a rural area of Puducherry. Prior to data collection permission was obtained from Directorate of Health and Family Welfare Services for conducting the main study. 30 antenatal mothers who fulfill the inclusion criteria were selected by purposive sampling technique. The data was collected using structured interview schedule on knowledge regarding antenatal care and 5 point likert scale to assess the attitude regarding antenatal care. Results: In pre test 18(60%) had Inadequate knowledge, 12 (40%) had moderately adequate Knowledge and none of them had adequate knowledge. In post-test, 18 (60%) had moderately adequate Knowledge, 12(40%) had adequate knowledge and none of them had inadequate knowledge. In pre test 3(10%) had neutral attitude, 27 (90%) had desirable attitude. In Post-Test 30 (100.0%) had desirable attitude, none of them had neither undesirable nor neutral attitude. Video assisted teaching programme was effective in improving the level of knowledge (t= 20.924) and attitude (t=8.148) regarding antenatal care among antenatal mothers at p<0.001.There was weak correlation between knowledge and attitude ( r=0.143). There was a statistically significant association between level of knowledge with previous knowledge at p<0.05. There was no statistically significant association between level of attitude and socio demographic variables. Conclusion: The findings of the study revealed that there is a significant difference between the pre test and post test level of knowledge and attitude regarding Antenatal care among antenatal mothers at different time points of study period. Hence it is proved that video assisted teaching programme was effective in enhancing the level of knowledge and attitude of antenatal mothers regarding antenatal care. There is significant association between pre test level of knowledge and attitude on antenatal care with selected socio demographic variables.