Effect of l-ascorbic acid on methomyl induced alterations in the lipid content in different tissues of the freshwater bivalve, lamellidens marginalis (lamarck)

The present study investigates the effect of Methomyl induced alterations in lipid level of gills, gonads and digestive gland tissues and its possible recovery by treating with L-Ascorbic acid in the fresh water bivalve, Lamellidens marginalis after chronic exposure. The freshwater bivalve Lamellidens marginalis were exposed to chronic dose of Methomyl (35 PPM LC50/2 values of 96 hours) alone and in combination with 50mg/L L-ascorbic acid for 21 days. Percent lipid contents in the gills, gonads and digestive gland tissues of control bivalve, Lamellidens marginalis were 4.5460±0.1560, 5.7463±0.0984, 6.2547±0.0758 respectively. Percent lipid contents in the gills, gonads and digestive gland of bivalve, Lamellidens marginalis on Methomyl intoxication were 4.5654±0.0831, 5.7658±0.1877 +10.63, 7.1566±0.0564 +18.42 respectively. Percent lipid contents in the gills, gonads and digestive gland of bivalve, Lamellidens marginalis on exposure to Methomyl intoxication with 50mg/L L-ascorbic acid were 4.9025±0.0440 +17.76, 5.9541±0.0365 +14.23, 8.098±0.2341 +34.43 respectively. Lipid contents in the gills, gonads and digestive gland of Methomyl and Methomyl with 50mg/L L-ascorbic acid exposed bivalve, Lamellidens marginalis showed remarkable increase in lipid content as compared to control. The increased level of lipid was observed in digestive glands as compared to other tissues. Exposure to pesticide Methomyl in combination with 50 mg/L of L-ascorbic acid showed considerable increase in the lipid levels. The pre-exposed bivalves for 21 days exposure to chronic dose of Methomyl showed recovery in normal water and the lipid contents were decreased to 4.2679±1.0255 +23.76, 5.2986±0.0286 +35.72, 6.4794±0.0469 +25.47 while percent lipid contents in presence of 50mg/L of L-ascorbic acid were decreased to 4.0077±1.0544 +17.65, 5.0457±0.3769 +27.48, 6.00652±0.1638 +25.66 respectively. Lipid content was found to be more in different tissues exposed to Methomyl without ascorbic acid as compared to Methomyl with ascorbic acid. Fast recovery of percent lipid contents was observed in presence of L-ascorbic acid than the recovery in the normal freshwater. This study indicates the protective and curative property of the L-ascorbic acid against the Methomyl induced damage.