Cultural parameters influencing the production of antimicrobial metabolites by rhodococcus erythropolis vl-rk_05

A study has been undertaken to optimize the production of antimicrobial metabolites by Rhodococcus erythropolis VL-RK_05 isolated from Mango orchards of Krishna District region by using basal medium as yeast extract malt extract dextrose medium. Five-day old culture showed maximum antimicrobial metabolite production when grown at pH 7.0 and temperature 30oC. The productivity of the strain was enhanced by amending the medium with sucrose and tryptophan each at a concentration of 1% (w/v) and 0.05% K2HPO4. The antimicrobial metabolites produced under optimized conditions exhibited broad spectrum of antimicrobial activity against different Gram positive and Gram negative bacteria as well as fungi.